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Advanced Plant Biotechnology I [1]

Paper Code: 
24BOT421A
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
  1. To develop an understanding of latest trends and various tools and techniques of Plant Biotechnology for human welfare.
  2. To impart knowledge about the techniques of plant tissue culture
  3. To study haploid production
  4. To have a basic understanding of the plant genetic transformation
  5. To gain knowledge on Cryopreservation
  6. To know the application of plant tissue culture

 

Course Outcomes: 

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

24BOT

421A

 

Advanced Plant Biotechnology I

 

CO110: Proficiently analyze Plant Tissue Culture techniques & apply these skills to propagate, regenerate & genetically modify plants.

CO111: Apply direct rDNA technology and assess Agrobacterium-mediated plant transformation at a level that demonstrates understanding and application of gene transfer for biotechnological progress.

CO112: Evaluate and synthesize cutting-edge methodologies in PTC, including organ culture, synthetic seed production, and cryopreservation, within the context of Biotechnology.

CO113: Utilize the technology of Green house Cultivation combining theoretical & practical expertise to design and implement in plant acclimatization for Agriculture & Horticulture.

CO114: Analyze & integrate advance technology in Secondary Metabolite Production with culture systems, incorporating biotransformation processes to engineer novel compounds for pharmaceutical & industrial applications.

CO115: Contribute effectively in course-specific interaction.

Approach in teaching:

Interactive Lectures, Discussions, assignments, Demonstration

Learning activities for the students:

Self-learning assignments, Effective question, simulation, Seminars, Quiz

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

12.00
Unit I: 
Plant Tissue Culture

 

PTC lab, media preparation and handling, cell and plant culture, sterile techniques, totipotency, various plant tissue culture media and their composition. Micropropagation: organogenesis, somatic embryogenesis, axillary bud proliferation.                                                                                                                                            

 

12.00
Unit II: 
Recombinant DNA Technology

 

Cutting and joining DNA molecules, cloning vectors: plasmids, cosmids, bacteriophage vectors, phagemids, YAC, shuttle vectors, expression vectors, gene cloning, direct DNA transfer: particle bombardment, electroporation, liposome mediated transformation, macroinjection; Agrobacterium

12.00
Unit III: 
Organ Culture

 

Anther culture, mature and immature zygotic embryo culture, ovary culture, somaclonal and gametoclonal variations, triploid production, production of virus free plants. Tissue culture in germplasm conservation: Synthetic seeds, Cryopreservation.                                                                                                                                     

 

12.00
Unit IV: 
Greenhouse Hardening Unit

 

Greenhouse Construction, types of greenhouses, environmental control inside a greenhouse. Soilless media for propagating and growing nursery plants. Media components: Sand, peat moss, coco-peat, vermiculite, pumice, perlite synthetic plastic aggregate and composts. Preplanting treatments of soil and soil mixes: heat treatments, fumigation and chemicals. 

 

12.00
Unit V: 
Secondary metabolite production in culture

 

Biotransformation, factors affecting production of secondary metabolites, production of important drugs in culture- taxol, ginseng, morphine, codeine, vinblastine and vincristine, types of bioreactors (stirred tank, airlift, membrane type, immobilized cell bioreactors).

 

Essential Readings: 
  • Bhojwani, S.S. (2012). Plant Tissue Culture: Applications and Limitations.  Netherlands: Elsevier Science.
  • Razdan, M. K. (2003). Introduction to Plant Tissue Culture. United Kingdom: Science Publishers.
  • Bhojwani, S., Razdan, M. (1996). Plant Tissue Culture: Theory and Practice. Netherlands: Elsevier Science.
  • Soh, W. Y., and Bhojwani, S.S. (2013). Morphogenesis in Plant Tissue Cultures.  Germany: Springer Netherlands.
  • Dantu, P. K., Bhojwani, S. S. (2013). Plant Tissue Culture: An Introductory Text. India: Springer India.
  • Chawla, H. S. (2011). Introduction to Plant Biotechnology (3/e). Jersey: CRC Press.
  • Scott, N., Slater, A., Fowler, M. (2008). Plant biotechnology: the genetic manipulation of plants. United Kingdom: OUP Oxford.

 

References: 

Suggested Readings:

  • Benson, E.E. (2002). Plant Conservation Biotechnology.  United Kingdom: Taylor & Francis.
  • Agnès Ricroch, ‎Surinder Chopra, ‎Shelby J. Fleischer · Plant Biotechnology: Experience and Future Prospects. (2014). Germany: Springer International Publishing.
  • Brown, T. A. (2018). Genomes 4. United States: CRC Press.
  • Collin, H., Edwards, S. (1998). Plant Cell Culture. Singapore: BIOS Scientific Publishers.
  • Wilson, C. M., Meredith, C. P., Hollaender, A., Kosuge, T. (2012). Genetic Engineering of Plants: An Agricultural Perspective. United States: Springer US.
  • Gyana Ranjan Rout, ‎K.V. Peter · 2018 (Ed.). Genetic Engineering of Horticultural Crops. (2018). United Kingdom: Elsevier Science.
  • Roberts, L., Staff, N. R. C. (1984). Genetic Engineering of Plants: Agricultural Research Opportunities and Policy Concerns. United States: National Academies Press.
  • Arencibia, A. (2000). Plant Genetic Engineering: Towards the Third Millennium. Netherlands: Elsevier Science.
  • John H. Dodds, ‎John Hamilton Dodds ·Plant Genetic Engineering. (1985). India: Cambridge University Press.

 

e-Resources:

 

Journals:

  • Plant Cell, Tissue and Organ Culture (Springer)
  • In Vitro Cellular and Developmental Biology- Plant (Springer)
  • Plant Biotechnology Journal (Wiley Online Library)
  • Plant Biotechnology Reports (Springer)

 

Academic Year: 
2024-2025 [12]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/advanced-plant-biotechnology-i-9

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/advanced-plant-biotechnology-i-9
[2] https://nptel.ac.in/courses/102106053
[3] https://www.researchgate.net/publication/291056431_General_Techniques_of_Plant_Tissue_Culture
[4] https://www.researchgate.net/publication/347914734_Green-houses_Types_and_Structural_Components
[5] https://www.usbg.gov/sites/default/files/usbg-greenhouse_manual.pdf
[6] https://www.researchgate.net/publication/344853645_PLANT_TISSUE_CULTURE_METHODS_IN_SECONDARY_METABOLITE_PRODUCTION_-A_MINI_REVIEW
[7] https://www.sciencedirect.com/topics/earth-and-planetary-sciences/biotransformation
[8] https://www.ancpatna.ac.in/departments/botany/lectures/UG-II/Manorma%20Kumari%20B,Sc,%20-II%20Embryo%20Culture.pdf
[9] https://chaudhary.kau.edu.sa/files/0030235/files/19046_lect%20recombinant%20dna%20tech_molecular%20genetics%20lect%202nd%20yr%20mt-1st%20semester.pdf
[10] https://facultystaff.richmond.edu/~lrunyenj/bio554/lectnotes/chapter14.pdf
[11] https://www.researchgate.net/publication/327111668_Recombinant_DNA_Technology_and_its_Applications_A_Review
[12] https://botany.iisuniv.ac.in/academic-year/2024-2025