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Botany Lab I [1]

Paper Code: 
24BOT125
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 
  1. To demonstrate technical knowledge and develop skill set that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

 

Course Outcomes: 

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

24BOT125

 

Botany Lab I

 

CO25: Develop holistic competencies viv-a-vis verbal and written communication skills, which will promote curiosity, innovation, and a passion for research including critical thinking

CO26: Develop skills and design experiments to study cytology and molecular biology techniques.

CO27: Improve and maximize knowledge of Cyanobacteria, Algae & Bryophytes and their role in sustainability.

Evaluate & standardise protocols for experiments in microbiology, mycology & plant pathology & also propose solutions for disease management.

CO28: Contribute effectively in course-specific interaction.

Approach in Teaching:

Substantial laboratory-based practical components and experiments Learning Activities for the students:

Peer teaching, Model preparation,

Field-based learning

Project-based learning

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce iinteractions are majorly adopted assessment methods

 

Unit I: 
Cell & Molecular Biology
 
  • Isolation of RNA from Yeast
  • Restriction Digestion
  • Agarose – Gel Electrophoresis
  • SDS- PAGE
  • Isolation of DNA from plant material

 

Unit II: 
Cytology & Cytogenetics

 

  • Study of general cytological techniques
  • Study of various stages of mitosis in Onion root tips
  • Study of various stages of meiosis in Onion flower bud
  • Calculation of mitotic index
  • To study B chromosomes in plants
  • Study of pollen viability
  • To measure different types of plant cells using Stage micrometer and Ocular micrometer
  • Induction of polyploidy in plants by treating shoot apical meristem with colchicine solution

 

Unit III: 
Hierarchy of Plant Kingdom

 

  • Preparation of report on Indian contributors in the field of Phycology and Bryology
  • Microscopic examination of stained cell preparation of Cyanobacteria by various
  • Staining  methods
  • Microscopic  study of representative members of Algae and Bryophytes.
  • Estimation of protein content in Spirulina, the Single Cell Protein
  • Study of different types of lichen thalli and their role in succession
  • List some Palaeobotanical evidences of members belonging to Algae and Bryophytes

 

Unit IV: 
Microbiology, Fungi & Plant Pathology

 

  • Cleaning of glassware
  • To study different methods of sterilization and disinfection by physical agents.
  • Instrumentation
  • To study various types of culture media
  • To determine the growth curve in bacteria (E.coli)
  • To study the bacteria by Gram staining
  • To become acquainted with Kirby-Baur procedure
  • Wine production by fermentative activities of Yeast cells
  • Isolation of microorganisms from the mixed culture by spread plate methods
  • Isolation of microorganism by pour plate method
  • To study ecological relationship between microorganisms of soil by Wino Gradsky Column
  • Survey of dermatological diseases caused by Fungi
  • Inventory preparations of antibiotics procured from Fungi
  • Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
  • Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
  • Report on eminent contributors in Mycology, Plant pathology and important related institutes.

 

References: 

Books Recommended:

  • Santra, SC and Chatterjee. 2006. College Botany Practical Vol. I. New Central Book Agency (P) Ltd., Calcutta.
  • Cavers, F. (2008). Practical botany. Whitefish, MT: Kessinger Pub.
  • Bendre, A. (2015). Practical Botany. S.L.: Rastogi Publications.
  • Santra, S.C. (2010). College Botany Practical Vol. II. New Central Book Agency (P) Ltd.

 

e-Resources:

 

Academic Year: 
2024-2025 [13]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/botany-lab-i

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/botany-lab-i
[2] https://microbiologyinfo.com/gram-staining-principle-procedure-interpretation-examples-and-animation/
[3] https://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol346_W06/labman_week4.pdf
[4] https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_II/Lab_03%3A_Obtaining_Pure_Cultures_from_a_Mixed_Population
[5] https://www.youtube.com/watch?v=nViO9Y4Yxfk
[6] https://www.youtube.com/watch?v=KFZYeApJ1Ls
[7] https://www.youtube.com/watch?v=nk37v3AXlbc
[8] https://www.youtube.com/watch?v=BhXoT0TGNsI
[9] https://www.youtube.com/watch?v=bm99zrq3ijo
[10] https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/04%3A_Staining_Techniques/4.03%3A_Lab_Procedures-_Bacterial_Smear_Simple_and_Gram_Staining
[11] https://www.youtube.com/watch?v=N-nIHhncEao
[12] https://www.youtube.com/watch?v=9HwPCb2QdCk
[13] https://botany.iisuniv.ac.in/academic-year/2024-2025