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PLANT BIOTECHNOLOGY-P [1]

Paper Code: 
24DBOT602A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

To develop skills related to plant tissue culture and transgenes

Course Outcomes: 

Course

Learning Outcome

(at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course title

24DBOT

602A

 

 

PLANT BIOTECHNOLOGY-P

 

 

CO57: Develop holistic competencies vis a vis verbal & written communication skill which will promote curiosity, innovation and a passion for research including critical thinking.

CO58: Apply the knowledge of PTC like media formulation, sterilization & culture techniques to achieve in vitro conservation

CO59: Analyse & integrate advance technology of biotransformation in culture systems to obtain a standardized protocol for scale-up production of commercially important compounds.

CO60: Contribute effectively in course-specific interaction. 

Approach in teaching: Interactive Lectures, Discussion, Demonstration, Team teaching, Substantial laboratory-based practical component and experiments,

Technology-enabled learning

Learning activities for the student:

Self-learning assignment, Effective questions, Stimulation, Seminar presentation

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods
 

 

CONTENTS:

  • Study of laboratory requirements and general techniques of biotechnology.
  • Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
  • Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
  • Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
  • Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
  • Demonstration of the technique of anther culture in Datura.
  • Demonstration of the technique of gel electrophoresis of bacterial DNA.
  • Demonstration of the phenomenon of action of Restriction Endonucleases.

 

Essential Readings: 
  • Archana, R. (2020). Practical Plant Biotechnology and Genetics. New Delhi: New India Publishing    Agency (NIPA.)
  • Kung, S., & Arntzen, C. J. (1989). Plant biotechnology. Boston: Butterworths.
  • Purohit, S. S. (2007). Practical plant biotechnology. Jodhpur: Student Edition.
  • Ashok M. BendreAshok Kumar. (2010). A Textbook of Practical Botany. Rastogi Publications.
  • Kurnaz, I. A. (2015). Techniques in Genetic Engineering. United Kingdom: CRC Press.
  • Nagar, S., Adhav. M. (2009). Practical Biotechnology and Plant Tissue Culture: S. Chand Limited.
  • Sherathiya, H. (2013). Practical Manual for Plant Tissue Culture: Basic Techniques of Plant Tissue Culture and Molecular Biology. Germany: GRIN Verlag.
  • Pllaiah, T., Subba Rao, M.V., Sreedevi, E. (2017). Plant Tissue Culture: Theory and Practicals. Scientific Publishers.
  • Reinert, J., Yeoman, M. (2012). Plant Cell and Tissue Culture: A Laboratory Manual. Germany: Springer Berlin Heidelberg.
  • Smith, R.H. (2012). Plant Tissue Culture: Techniques and Experiments. Academic Press.

 

References: 

 

 

 

Academic Year: 
2024-2025 [6]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/plant-biotechnology-p

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/plant-biotechnology-p
[2] http://www2.southeastern.edu/Academics/Faculty/jtemple/486/experiment%202.pdf
[3] https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.12%3A_Restriction_Digest_with_Gel_Electrophorisis
[4] https://archive.nptel.ac.in/noc/courses/noc17/SEM2/noc17-bt21/
[5] http://amrita.olabs.edu.in/
[6] https://botany.iisuniv.ac.in/academic-year/2024-2025