Published on Botany (https://botany.iisuniv.ac.in)

Home > Practical

Practical [1]

Paper Code: 
DBOT 602A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to-

  1. To develop skills related to plant tissue culture and transgenes is.

 

Course Outcomes (COs):

Course Outcomes

Learning and teaching strategies

Assessment Strategies

 
 

On completion of this course, the students will be able to:

CO58: Apply the knowledge of good laboratory practices in Plant Biotechnology.

CO59: Design various tissue culture experiments for plant propagation.

CO60: Utilize different tools and techniques of modern science in research.

CO61: Gain technical know-how about the patent application process.

 

 

 

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods

  
 

 

  • Study of laboratory requirements and general techniques of biotechnology.
  • Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
  • Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
  • Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
  • Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
  • Demonstration of the technique of anther culture in Datura.
  • Demonstration of the technique of gel electrophoresis of bacterial DNA.
  • Demonstration of the phenomenon of action of Restriction Endonucleases.

 

 

Essential Readings: 
  • Archana, R. (2020). Practical Plant Biotechnology and Genetics. New Delhi: New India Publishing    Agency (NIPA.)
  • Kung, S., & Arntzen, C. J. (1989). Plant biotechnology. Boston: Butterworths.
  • Purohit, S. S. (2007). Practical plant biotechnology. Jodhpur: Student Edition.
  • Ashok M. BendreAshok Kumar. (2010). A Textbook of Practical Botany. Rastogi Publications.
  • Kurnaz, I. A. (2015). Techniques in Genetic Engineering. United Kingdom: CRC Press.
  • Nagar, S., Adhav. M. (2009). Practical Biotechnology and Plant Tissue Culture: S. Chand Limited.
  • Sherathiya, H. (2013). Practical Manual for Plant Tissue Culture: Basic Techniques of Plant Tissue Culture and Molecular Biology. Germany: GRIN Verlag.
  • Pllaiah, T., Subba Rao, M.V., Sreedevi, E. (2017). Plant Tissue Culture: Theory and Practicals. Scientific Publishers.
  • Reinert, J., Yeoman, M. (2012). Plant Cell and Tissue Culture: A Laboratory Manual. Germany: Springer Berlin Heidelberg.
  • Smith, R.H. (2012). Plant Tissue Culture: Techniques and Experiments. Academic Press.

 

 

References: 

e-Resources:

 

Academic Year: 
2023-2024 [6]

Follow Botany on:

IIS(deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158

Source URL: https://botany.iisuniv.ac.in/courses/subjects/practical-68

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/practical-68
[2] http://www2.southeastern.edu/Academics/Faculty/jtemple/486/experiment%202.pdf
[3] https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.12%3A_Restriction_Digest_with_Gel_Electrophorisis
[4] https://archive.nptel.ac.in/noc/courses/noc17/SEM2/noc17-bt21/
[5] http://amrita.olabs.edu.in/
[6] https://botany.iisuniv.ac.in/academic-year/2023-2024