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Tools and Techniques of Plant Science. [1]

Paper Code: 
BOT 323
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objectives:

This course will enable the students to –

  1. The course aims at studying various novel techniques that have become an integral part of learning and understanding plant systems.

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 323

 

Tools and Techniques of Plant Science

On completion of this course, the students will be able to:

CO67:  Classify different types of chromatography techniques.

CO68: Explain the principles of light microscopy, compound microscopy, fluorescence microscopy and confocal microscopy

CO69:  Apply and compare suitable strategies for data collections and disseminating research findings.

CO70:  Summarize the methods of Biostatistics and its application in Biology.

CO71: Execute and implement practical knowledge for employment in research labs associated with biosciences

Class lectures

Seminars

Tutorials

Group discussions and Workshops

Question preparation

 

 

 

Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects
 

 

12.00
Unit I: 
Biostatistics

Biostatistics: Scope of Biostatistics, exploring and presenting data: scales of measurement (nominal and ordinal), tables and graphs for nominal, ordinal and numerical data, summarizing data: measure of central tendency and dispersion    

12.00
Unit II: 
Microscopy

Microscopy: Magnifiers and microscopes, simple and compound microscopes, bright field and dark field microscopy, confocal and deconvolution microscopy, microscopic measurements, phase contrast, fluorescence microscopy, electron microscopy – SEM, TEM.            

12.00
Unit III: 
Histology

Histology of Tissues: Selection of material, chemical fixation – types of fixatives, mechanism of fixation, tissue dehydration – general protocol, rapid dehydration and dehydration using a graded solvent series of ethanol or acetone, infiltrating and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy: cryotome and vibratome.

 

12.00
Unit IV: 
Basic Techniques

Basic Techniques: Agarose gel electrophoresis, SDS-PAGE, isolation and purification of genomic DNA, elution from gel, Blotting (Southern, Northern and Western), HPLC, Autoradiography.

 

12.00
Unit V: 
Microbiological and Immunotechniques:

Microbiological and Immunotechniques: Sterilization and disinfection: Preparation of media, sterilization, culture and transfer instruments, cultivation chambers, culture transfer techniques, pure culture techniques: streak plate, pour plate and spread plate method, media preparation-solid and liquid media, simple and complex media, nutrient agar slabs, slants and plates, staining techniques, simple and differential staining, enumeration using haemocytometer, bio chemical characterization – TSI test, IMViC test, ELISA, Radio Immunoassay (RIA), Ouchterlony latex agglutination test, monoclonal and polyclonal antibody production

 

Essential Readings: 

Essential Readings:

  • Eberly, L. E., and Le, C. T. (2016). Introductory Biostatistics. Germany: Wiley.
  • Lee, E. S., and  Forthofer, R. N. (2014). Introduction to Biostatistics: A Guide to Design, Analysis, and Discovery. United States: Elsevier Science.
  • John Walker, and Keith Wilson. (2010). Principles and Techniques of Biochemistry and Molecular Biology.  United States: Cambridge University Press.
  • Cooper, G. M., Hausman, R. E. (2013). The Cell: A Molecular Approach. United States: Sinauer Associates.
  • Braun, R. D. (2016). Introduction to Instrumental Analysis. India: Pharma Med Press.
  • Clarkson, A. (2017). A Text-Book of Histology, Descriptive and Practical: For the Use of Students (Classic Reprint). United States: Fb&c Limited.
  • Erkmen, O. (2021). Laboratory Practices in Microbiology. Netherlands: Elsevier Science.
  • Maheshwari, D. K. (1999). A Textbook of Microbiology. India: S. Chand.
  • Banerjee, A.K. and Banerjee, N. (2008). Fundamentals Of Microbiology & Immunology.  India: New Central Book Agency (P) Limited.
  • Punt, J., Jones, P., Owen, J., and Stranford, S.  (2018).  Kuby Immunology. United States: Macmillan Learning.

 

Suggested Readings:

  • Indranil, S., and Paul, B., (2020).   Essentials Of Biostatistics & Research Methodology.  Academic Publishers.
  • Rao, G. N. (2018). Biostatistics & Research Methodology. India: PharmaMed Press.
  • Bancroft, J. D., Layton, C., and Suvarna, K. S. (2012). Bancroft's Theory and Practice of Histological Techniques E-Book. United Kingdom: Elsevier Health Sciences.
  • Benson, H. J. (2015). Benson's microbiological applications: Laboratory manual in general microbiology: Tata McGraw Hill
  •  Cappuccino, J.G. and Sherman, N. A. W. (2013). Microbiology, a Laboratory Manual. Pearson

 

References: 

e-Resources:

 

Journals:

  • Microbiological Research (Elsevier)
  • Indian Journal of Medical Microbiology (Elsevier)
  • Environmental Microbiology (Wiley)
  • Histopathology (Wiley)
  • Nature Reviews Microbiology (Nature)

 

 

Academic Year: 
2023-2024 [12]

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[1] https://botany.iisuniv.ac.in/courses/subjects/tools-and-techniques-plant-science-3
[2] https://onlinecourses.nptel.ac.in/noc22_bt13/preview
[3] https://onlinecourses.swayam2.ac.in/cec22_bt02/preview
[4] https://onlinecourses.nptel.ac.in/noc22_cy29/preview
[5] https://archive.nptel.ac.in/noc/courses/noc22/SEM1/noc22-bt13/  
[6] https://nptel.ac.in/courses/102101050
[7] https://nptel.ac.in/courses/102101056
[8] https://nptel.ac.in/courses/102108082
[9] https://www.researchgate.net/publication/332058163_Notes_on_Histological_Techniques
[10] https://www.researchgate.net/publication/320945390_Introduction_to_Microscopy
[11] https://www.imperial.ac.uk/media/imperial-college/medicine/facilities/film/Basics-website.pdf
[12] https://botany.iisuniv.ac.in/academic-year/2023-2024