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Practical [1]

Paper Code: 
BOT 603
Credits: 
2
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives 

This course will enable the students to-

  1. To develop skills related to plant tissue culture and applied botany vis.a.vis horticulture, forestry and gardening.

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 603

 

Practical

On completion of this course, the students will be able to;

CO95:  Acquire hands on training on different techniques of plant propagation such as cutting, grafting and layering.

CO96: Explain and design various tissue culture experiments for plant propagation

CO97: Implement practical knowledge of various garden operations.

CO98: Integrate and attributing role of plants in cosmetic industry, medicine, environmental cleanup and energy sector

 

 

Substantial laboratory-based practical component and experiments

 

Technology-enabled learning

 

Field-based learning

Project-based learning

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

Viva voce interviews are majorly adopted assessment methods
 

 

  • Study of laboratory requirements and general techniques of biotechnology.
  • Principle and application of the following- Laminar Air Flow, Autoclave and Ultracentrifugation.
  • Preparation of different types of tissue culture medias and solutions of nutrients and growth regulators
  • Demonstration of inoculation technique, asceptic transfer of explant, optimizing the conditions for direct/ indirect differentiation in different explants to study totipotency, cytodifferentiation and organogenesis.
  • Germination of pollen of Catharanthus roseus in 10 % sucrose solution.
  • Demonstration of the technique of protoplast isolation and somatic embryogenesis in Daucus carota.
  • Demonstration of the technique of anther culture in Datura.
  • Demonstration of the technique of gel electrophoresis of bacterial DNA.
  • Demonstration of the phenomenon of action of Restriction Endonucleases. Field study of various plant forms- climbers, trees, shrubs, orchids, ferns and palms.
  • Experimental study of the technique of vegetative propagation-cutting, budding, grafting and air layering etc.
  • To study propagation by means of bulbs, corms, tubers, rhizome etc.
  • To study different styles of garden design.
  • Field visit to understand different patterns of landscape design.
  • Preparation of record on local biodiversity.

 

 

 

 

Essential Readings: 
  • Trivedi, P. C. (2005). Applied botany. Jaipur, India: Aavishkar Publishers.
  • Trivedi, P. P. (2010). Home gardening. New Delhi: Indian Council of Agricultural Research.
  • Percy L. (2013). Gardening in India.Taylor and Francis, England
  • Archana, R. (2020). Practical Plant Biotechnology and Genetics. New Delhi: New India Publishing    Agency (NIPA.)
  • Kung, S., & Arntzen, C. J. (1989). Plant biotechnology. Boston: Butterworths.
  • Purohit, S. S. (2007). Practical plant biotechnology. Jodhpur: Student Edition.
  • Ashok M. BendreAshok Kumar. (2010). A Textbook of Practical Botany. Rastogi Publications.
  • Pitman, V., (2004). A practical book of Aroma therapy: Nelson Thornes Publishers
  • Rexford, E. E. (2015). Home Floriculture: A Practical Guide to the Treatment of Flowering and Other Ornamental Plants in the House and Garden. United States: 1kg Limited.
  • Kurnaz, I. A. (2015). Techniques in Genetic Engineering. United Kingdom: CRC Press.
  • Nagar, S., Adhav. M. (2009). Practical Biotechnology and Plant Tissue Culture: S. Chand Limited.
  • Sherathiya, H. (2013). Practical Manual for Plant Tissue Culture: Basic Techniques of Plant Tissue Culture and Molecular Biology. Germany: GRIN Verlag.
  • Pllaiah, T., Subba Rao, M.V., Sreedevi, E. (2017). Plant Tissue Culture: Theory and Practicals. Scientific Publishers.
  • Reinert, J., Yeoman, M. (2012). Plant Cell and Tissue Culture: A Laboratory Manual. Germany: Springer Berlin Heidelberg.
  • Smith, R.H. (2012). Plant Tissue Culture: Techniques and Experiments. Academic Press.

 

References: 

e-Resources:

 

Academic Year: 
2022-2023 [6]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/practical-54

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/practical-54
[2] http://www2.southeastern.edu/Academics/Faculty/jtemple/486/experiment%202.pdf
[3] https://bio.libretexts.org/Bookshelves/Biotechnology/Lab_Manual%3A_Introduction_to_Biotechnology/01%3A_Techniques/1.12%3A_Restriction_Digest_with_Gel_Electrophorisis
[4] https://archive.nptel.ac.in/noc/courses/noc17/SEM2/noc17-bt21/
[5] http://amrita.olabs.edu.in/
[6] https://botany.iisuniv.ac.in/academic-year/2022-2023