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Practical [1]

Paper Code: 
BOT 125
Credits: 
8
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Objective:

This course will enable the students to –

  1. To demonstrate procedural knowledge that creates different types of professionals in the field of Botany like in research and development, teaching government and public services. 

Course Outcomes (COs):

     Course

Course Outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

BOT 125

 

Practical

 

On completion of this course, the students will be able to:

CO21: Understand the microscopic techniques and familiarize students with the external and internal structure of lower group organisms.

CO22: Apply the knowledge to microscopic preparations and subsequent observation of the permanent slides of genera.

CO23: Understand the basic concept of DNA isolation, SDS-PAGE and gel electrophoresis.

CO24: Analyse the difference of structure in the prescribed groups of microbes and plants.

CO25: Understand the different methods of Staining used in Identification of forms.

 

 

Substantial laboratory-based practical component and experiments

Technology-enabled learning

Field-based learning

Project-based learning

 

 

Practical assignments and laboratory reports

Observation of practical skills

Individual and group project reports

 

Viva voce interviews are majorly adopted assessment methods
 

 

Unit I: 
BOT 121: Cell & Molecular Biology
  • Isolation of RNA from Yeast
  • Restriction Digestion
  • Agarose – Gel Electrophoresis
  • SDS- PAGE
  • Isolation of DNA from plant material

 

Unit II: 
BOT 122: Cytology & Cytogenetics
  • Study of general cytological techniques
  • Study of various stages of mitosis in Onion root tips
  • Study of various stages of meiosis in Onion flower bud
  • Calculation of mitotic index
  • To study B chromosomes in plants
  • Study of pollen viability
  • To measure different types of plant cells using Stage micrometer and Ocular micrometer
  • Induction of polyploidy in plants by treating shoot apical meristem with          colchicines solution

 

Unit III: 
BOT 123: Hierarchy of Plant Kingdom
  • Preparation of report on Indian contributors in the field of Phycology and Bryology
  • Microscopic examination of stained cell preparation of Cyanobacteria by various
  • Staining  methods
  • Microscopic  study of representative members of Algae and Bryophytes.
  • Estimation of protein content in Spirulina, the Single Cell Protein
  • Study of different types of lichen thalli and their role in succession
  • List some Palaeobotanical evidences of members belonging to Algae and Bryophytes

 

Unit IV: 
BOT 124: Microbiology, Fungi & Plant Pathology
  • Cleaning of glassware
  • To study different methods of sterilization and disinfection by physical agents.
  • Instrumentation
  • To study various types of culture media
  • To determine the growth curve in bacteria (E.coli)
  • To study the bacteria by Gram staining
  • To become acquainted with Kirby-Baur procedure
  • Wine production by fermentative activities of Yeast cells
  • Isolation of microorganisms from the mixed culture by spread plate methods
  • Isolation of microorganism by pour plate method
  • To study ecological relationship between microorganisms of soil by Wino Gradsky Column
  • Survey of dermatological diseases caused by Fungi
  • Inventry preparations of antibiotics procured from Fungi
  • Morphological study of representative members of Fungi- Pilobolus, Chaetomium, Morchella, Melampsora, Polyporus, Dreschlera, Phoma, Peziza, Puccinia
  • Symptomatology, identification, causal organism of the following diseases: White rust, bunt, smut, green ear, smut and ergot of Bajra, rust and smut of wheat, paddy blast, cotton wilt, downy mildew and powdery mildew of grapes, tundu, tobacco mosaic virus, little leaf of brinjal, root knot of vegetables
  • Report on eminent contributors in Mycology, Plant pathology and important related institutes

 

 

Essential Readings: 

Books Recommended:

  • Santra, SC and Chatterjee. 2006. College Botany Practical Vol. I. New Central Book Agency (P) Ltd., Calcutta.
  • Cavers, F. (2008). Practical botany. Whitefish, MT: Kessinger Pub.
  • Bendre, A. (2015). Practical Botany. S.L.: Rastogi Publications.
  • Santra, S.C. (2010). College Botany Practical Vol. II. New Central Book Agency (P) Ltd.

 

References: 

e-Resources:

 

Academic Year: 
2022-2023 [13]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/practical-46

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/practical-46
[2] https://microbiologyinfo.com/gram-staining-principle-procedure-interpretation-examples-and-animation/
[3] https://fire.biol.wwu.edu/cmoyer/zztemp_fire/biol346_W06/labman_week4.pdf
[4] https://bio.libretexts.org/Learning_Objects/Laboratory_Experiments/Microbiology_Labs/Microbiology_Labs_II/Lab_03%3A_Obtaining_Pure_Cultures_from_a_Mixed_Population
[5] https://www.youtube.com/watch?v=nViO9Y4Yxfk
[6] https://www.youtube.com/watch?v=KFZYeApJ1Ls
[7] https://www.youtube.com/watch?v=nk37v3AXlbc
[8] https://www.youtube.com/watch?v=BhXoT0TGNsI
[9] https://www.youtube.com/watch?v=bm99zrq3ijo
[10] https://bio.libretexts.org/Courses/North_Carolina_State_University/MB352_General_Microbiology_Laboratory_2021_(Lee)/04%3A_Staining_Techniques/4.03%3A_Lab_Procedures-_Bacterial_Smear_Simple_and_Gram_Staining
[11] https://www.youtube.com/watch?v=N-nIHhncEao
[12] https://www.youtube.com/watch?v=9HwPCb2QdCk
[13] https://botany.iisuniv.ac.in/academic-year/2022-2023