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Practical [1]

Paper Code: 
BOT 423
Credits: 
9
Contact Hours: 
60.00
Max. Marks: 
100.00
  • Media preparation, sterilization and inoculation of given material.
  • Growth characteristics of E.coli using plating and turbidimetric methods.
  • Isolation of plasmid from E.coli by alkaline lysis method and its quantitation spectrophotometrically.
  • Restriction digestion of the plasmid and estimation of the size of various DNA fragments.
  • Cloning of a DNA fragment in a plasmid vector, transformation of the given bacterial population and selection recombinants.
  • Organogenesis and somatic embryogenesis using appropriate explantsand preparation of artificial seeds.
  • Isolation of protoplast from various plant tissues and testing their viability.
  • Effect of physical and chemical factors protoplat yield.
  • Demonstration of protoplast fusion employing PEG.
  • Demonstration of DNA sequencing using Sanger,s dideoxy method.
  • Demonstration of microtechniques for plant cultures.
  • Co-cultivation of the plant material with Agrobacterium and study GUS activity histochemically.

 

 

Academic Year: 
2014-2015 [2]

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Source URL: https://botany.iisuniv.ac.in/courses/subjects/practical-35

Links:
[1] https://botany.iisuniv.ac.in/courses/subjects/practical-35
[2] https://botany.iisuniv.ac.in/academic-year/2014-2015